Free Radical Scavenging Activity and Cytotoxic Effect of Anisaldehyde on Human Cancer Cell Lines

The present investigation was an attempt to evaluate the antioxidant and anticancer property of Anisaldehyde in vitro. Anisaldehyde is an organic compound that consists of a benzene ring substituted with an aldehyde and a methyl group. It is very effective as a carminative (to relieve gas pains). In this study, free radical scavenging activity was evaluated using DPPH (α,α-diphenyl-β-crylhydrazyl), Iron chelating, Reducing power assay and the cytotoxicity was assessed by MTT method using human cancer cell lines. The lowest DPPH free radical scavenging activity of Anisaldehyde obtained was 20% at 400 μg/ml and the highest DPPH free radical scavenging activity obtained was 21% at 800μg/ml concentrations.Maximum chelation of metal ions obtained was 27% at 1000 μg/ml and the reductive capability was high in 23% at 1000 μg/ml. The Anti-cytotoxic activity was evaluated against various cancer cell lines such as human breast adrenocarcinoma cell line (MCF 7), liver adrenocarcinoma (HepG2) and epidermoid carcinoma (ME 180). The IC50 values of 400,600 and 800μg/ml at MCF 7, HepG2 and ME 180 cell lines respectively. In this result the Anisaldehyde showed a superior effect on MCF 7 compared to other cell lines. Therefore, the Anisaldehyde proved to be a potent antioxidant and anticancer property against human cancer cell lines.